There are good bus connections from Orlando to all the major cities in Florida; unfortunately, the bus station is way out of town. By the time you've factored in the taxi ride, you might be just as well to take a train. Amtrak runs direct services between Orlando and Miami, Gainesville and points to the north. Or you can just fly straight into Orlando International Airport, which is served by almost all major airlines, as well as charters and discount airlines.
Bus
The bus station is in the middle of nowhere out on Hwy 423, but the Greyhound buses will take you to every major city in Florida. If you're arriving by bus, factor in the cost of an expensive cab ride into town.
Plane
Orlando International Airport (MCO), in the far southeastern corner of the city, is the largest in Central Florida. It's served by almost all major airlines, as well as charters and discount airlines. If you get to Florida by package deal you'll inevitably end up in Orlando, roped in by cross-marketing plans between theme parks, hotels and airlines. To get to Disney World from the airport by car, take Hwy 417 to Hwy 536.
Train
Amtrak runs direct services from Orlando to Miami, Gainesville and points to the north. The Amtrak station is 1.6km (1mi) south of Central Orlando.